Activities concerning the WP2 (metabolic engineering for biomass conversion to butanol) have been carried out by WEIZMANN.

A library of lignocellulolytic enzymes has been developed and a panel of mixtures tested on the four different types of untreated agro-wastes.

High levels of enzymatic degradation were observed on brewer’s spent grain by a mixture of 3 cellulases, 1 xylanase and 1 bifunctional xylanase-laccase.

These five enzymes were attached to scaffolding to form a designer cellulosome complex, which was found to be even more efficient than the free enzyme mixture.